the prevalence of toxoplasma infection among free-ranging chickens in southern iran using ifa and nested-pcr
نویسندگان
چکیده
background: as consumption of chicken meat may be as one of the sources of human infection, this study was undertaken to determine the prevalence of t. gondii in farm chickens ( gallus gallus domesticus ) in shiraz, southern iran. methods: two hundred and thirty one blood samples were collected from farm chickens by a cluster random sampling method and tested for toxoplasmosis by indirect fluorescent antibody technique (ifat). the samples of the brain, heart, and liver of the chickens were tested by a nested pcr method. the results were analyzed by spss software using chi-square test and a p value <0.05 was considered statically significant. results: out of 58 seropositive chickens, 29 (1:16 in eight, 1:32 in 14, 1:64 in five and 1:128 in two birds) and out of seronegative chickens, three were enrolled in the study. the most infected tissue was liver (27 out of 29) and the lowest was the heart (16 out of 29) ( α =0.05, p =0.002). none of the seronegative chickens was positive in pcr method. only 2 out of 8 cases with a titer of 1:16 (as cut off point) were negative in pcr method whereas the remained were positive. conclusion: based on cultural and food habits in our area, the meat and viscera of chicken may be important sources of infection in human when consuming semi-cooked meats. considering the high prevalence of toxoplasmosis in chickens, standards in chicken breeding, education of environmental health personnel and standardization for preparation and handling techniques are required by health and veterinary organizations.
منابع مشابه
Prevalence of Food-Borne Toxoplasma gondii in Free-Ranging Chickens Sold in Tunis, Tunisia
Background: Toxoplasma gondii, as an important food-borne protozoan parasite, occurs widely in humans and animals including domestic poultry throughout the world. The goal of this survey was to determine prevalence rate of T. gondii in free-ranging chickens sold in Tunis, Tunisia. Methods: In the present study, the prevalence of T. gondii in 40 free-range chickens (Gallus domesticus) marketed ...
متن کاملprevalence of food-borne toxoplasma gondii in free-ranging chickens sold in tunis, tunisia
background: toxoplasma gondii, as an important food-borne protozoan parasite, occurs widely in humans and animals including domestic poultry throughout the world. the goal of this survey was to determine prevalence rate of t. gondii in free-ranging chickens sold in tunis, tunisia. methods: in the present study, the prevalence of t. gondii in 40 free-range chickens (gallus domesticus) marketed i...
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متن کامل
Toxoplasma gondii infection in domestic ducks, free-range and caged chickens in southern China.
Toxoplasma gondii is widely distributed in humans and other animals including domestic poultry throughout the world, but little is known of the prevalence of T. gondii in chickens and ducks in People's Republic of China. In the present study, antibodies to T. gondii were investigated in 349 domestic ducks (Anas spp.), 361 free-range, and 244 caged chickens (Gallus domesticus) raised in commerci...
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عنوان ژورنال:
iranian journal of parasitologyجلد ۴، شماره ۴، صفحات ۲۹-۳۶
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